P-147 Comparison of Fertilization rate, Blastocyst rate and number of Top Quality Embryo with Short (2 hours) and conventional co-incubation (16-20 hours)of gametes

Abstract Study question Does short Co-incubation of gametes increase quality and quantity embryos by eliminating suboptimal culture conditions like increased reactive oxygen species seen with overnight incubation? Summary answer Although co-incubation group had lower fertilization rate it yielded more blastocysts top on day 3 5. What is known already Successful can occur 20 minutes after gametes. Incubation time 18 hours has been used as a routine combining check. However to produce (ROS) released cellular metabolism inseminated sperms which affect embryo development cause zona pellucida hardening. Studies have shown reduced oocyte exposure caused improved development. However, other studies reported no such advantage. Not many done without denudation might allow optimum at the same prevent in ROS. design, size, duration It prospective controlled study. 62 patients (921 oocytes) were recruited. The sibling oocytes from each patient divided into 2 groups. Group 1( Control) conventional (16-20 hours) (2 they transferred fresh media. period was January 2022 September 2022. Institutional scientific ethical committee approval written consent couple taken. Participants/materials, setting, methods Couples undergoing IVF tertiary infertility centre Women under 40 years who minimum 6 males normal semen parameters included. Those excluded couples requiring ICSI, poor responders <6 retrieved requesting transfer. Primary outcome number percentage (TQE) blastocyst rate. Secondary SPSS for data analysis. Results expressed mean +/-SD or Main results role chance Total studied 921 patients. Since control study matched age BMI, ovarian reserve, stimulation protocol, laboratory handling. Extra odd randomized Mean 31 years. 1 425 out 455 (93.4%) fertilized 412 466 (88.4%) (p value<0.05). TQE 251 (59.05%) 277 (67.23%) = 0.013). Blasts formed 41 (9.64%) were135 (32.76%) 0.001). 5 28/425 (6.67%) (0.45 +/−0.9 blasts per patient) 95/412 (23.05%) (1.53+/-1.86 patient). (P 0.001) Percentage grade significantly higher group. did not progress beyond both less absolute value this Limitations, reasons caution Pregnancy could be calculated some wanted one only single blast. For their transfer taken Very (<6 studied. Further needed establish best (1-8 Wider implications findings Practice 16-20 needs re-evaluated. shows insemination period. This may become practice if established further assuming that are yield better pregnancy rates Trial registration applicable